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Sequences and Domain Structures of Mammalian, Avian, Amphibian and Teleost Tropoelastins
The general arrangement of alternating hydrophobic and crosslinking domains that is a prominent characteristic of human, mouse and chicken tropoelastins (Fig. 4) is also clearly present in tropoelastins from zebrafish and xenopus (Fig. 5).In most cases domains correspond to exons, such that designations of exons as either hydrophobic or crosslinking are straightforward.
Local Micromechanical Properties of Decellularized Lung Scaffolds Measured With Atomic Force Microscopy
Mechanical properties of acellular lung tissue have been probed in air-filled decellularized murine lungs by recording quasi-static pressure-volume curves during inflation and deflation [34] and by measuring respiratory impedance with the forced oscillation technique [46].This approach does not provide accurate estimation of lung parenchymal ECM, since mechanical measurements performed on ...
Matrix Preparation, Reseeding Technique and Short-Term Implantation in a Porcine Model
Reconstruction of the urinary tract using the principles of tissue engineering has been a major focus of experimental research in latter years. 1 Different acellular matrices or scaffolds reseeded with viable cells are the 2 main options for this kind of tissue replacement. In each case the ideal matrix should be biocompatible, bioabsorbable, nonimmunogenic and able to support cell attachment ...
Bioreactor Design for Perfusion-Based, Highly Vascularized Organ Regeneration
The premise of bioartificial organs is to strip an organ that is nontransplantable, due to parenchyma scarring or high fat content, of its cellular components using a process termed decellularization to yield a scaffold on which to develop a new organ (Figure 1).Important properties of these scaffolds are retention of native tissue architecture and maintenance of extracellular matrix (ECM ...
Three-Dimensional Filamentous Human Diseased Cardiac Tissue Model
A human in vitro cardiac tissue model would be a significant advancement for understanding, studying, and developing new strategies for treating cardiac arrhythmias and related cardiovascular diseases. We developed an in vitro model of three-dimensional (3D) human cardiac tissue by populating synthetic filamentous matrices with cardiomyocytes derived from healthy wild-type volunteer (WT) and ...