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RNA editing with CRISPR-Cas13 | Science - AAAS
Precise nucleic acid-editing technologies are valuable for studying cellular function and as novel therapeutics. Current editing tools, based on programmable nucleases such as the prokaryotic CRISPR-associated nucleases Cas9 (1-4) or Cpf1 (), have been widely adopted for mediating targeted DNA cleavage, which in turn drives targeted gene disruption through nonhomologous end joining (NHEJ ...
Repair of CRISPR-guided RNA breaks enables site ... - Science | AAAS
CRISPR-guided endonucleases have enabled programable DNA cleavage and the development of new therapeutics (1, 2).The first generation of CRISPR genome editing used Cas9 nuclease to make double-stranded DNA breaks that are repaired by the cell, leading to site-specific edits ().These technologies have enriched our mechanistic understanding of DNA repair and new insights into repair are used to ...